Dear Matthias, Have a look at this paper:
Large scale expression and purification of the mouse 5-HT3 receptor. Hassaine G, Deluz C, Li XD, Graff A, Vogel H, Nury H. Biochim Biophys Acta. 2013 Nov;1828(11):2544-52. Best, xiaodan -----Original Message----- From: CCP4 bulletin board on behalf of Matthias Zebisch Sent: Tue 1/7/2014 10:33 To: [email protected] Subject: [ccp4bb] Membrane protein structures from mammalian expression Dear crystallographers, happy new year to everybody! Can I ask you to send me examples where expression of fully integral membrane proteins in mammalian cells (not insect!) has led to the successfull determination of a crystal structure? Thanks very much, Matthias ----------------------------------------- Dr. Matthias Zebisch Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK Phone (+44) 1865 287549; Fax (+44) 1865 287547 Email [email protected] Website http://www.strubi.ox.ac.uk -----------------------------------------
