Do you use any Dnase while purifying your protein? if not try that and set up new drops to check again.
Sent from my iPhone On 13.01.2014, at 21:02, Debasish Chattopadhyay <[email protected]> wrote: > We crystallized a protein at 4 and 22 deg C in different conditions: > > from ammonium sulfate in acetate buffer pH 5 > and > PEG4000 in Hepes buffer at pH 7.5 > > In both cases the drops have a slimy skin (almost feels like DNA). We > therefore think that the skin is generated from the protein. > > I am sure some of you have had similar experiences. I would like your > suggestions about how to avoid the skin. Please note that we are not asking > for suggestions on how to handle the skin (such as using various tools) we > are only interested in knowing if there is a way to prevent the formation of > the skin. > > Thank you so much > > Debasish > > Ph: (205)934-0124; Fax: (205)934-0480 >
