Hi Chris, It would be nice to have a wee bit more information. Is 3.4-3.0 angstroms from a home source or synchrotron? What are the crystallization conditions for both the native and SeMet crystals? Did you see the SeMet crystals with the native crystals. Have you tried MMS with the native crystals into new screening conditions.
Cheers, Scott On Jan 18, 2014, at 12:14 PM, Chris Fage <cdf...@gmail.com> wrote: > Hello Everyone, > > I am currently trying to phase a structure with an asymmetric unit predicted > to contain 20-24 monomers (space group P1). The native crystals, while > beautiful in appearance (see attached), only diffract to ~3.4-3.0 angstroms > at best, and SeMet-derived crystals grow with poor morphology (small > needles). Also, based a fluorescence scan, I know that mercury does not bind > appreciably. Other than screening for a new space group, what options might I > have for phasing this many monomers at lower resolution? Is there any real > chance of solving the structure in this space group? > > Thank you in advance for any suggestions! > > Regards, > Chris > <Crystals.jpg>
