Hi ccp4bb,
I would like to perform an MS analysis of the protein crystals after
X-ray exposure. I'm interested in the characterization of the
modifications on the cofactor (expected mass cca 400Da). I will need to
wash the crystals before MS because they contain glycerol, long chain
PEG and detergent. I'm trying to find a way how to wash these molecules
out without losing the cofactor or breaking the crystal. As I don't have
many crystals, I was wondering if some of you have tried such analysis
and/or washing..
any advice would be greatly appreciated
roman
