Dear Theresa,
We offer the possibility for data collection at room temperature on our
beamline, FIP-BM30A at the ESRF, for many years. It's really routine now
(see http://www.fip-bm30a.fr/).
Data are collected in situ, on the crystallization plate, thanks to the
robotized system we developed for that (G-Rob, see
http://www.natx-ray.com). We have a ADSC 315r CCD detector. When symetry
is high enough, one crystal can be enough to get a complete dataset (up
to 80° total rotation is possible, with appropriate crystallization
plates).
The first structure solved in situ on our beamline on a single crystal
was the one of NDK from A. Polyphaga Mimivirus (C. Abergel, L.
Jacquamet, CNRS):
space group: p6(3), resolution: 2.3 Å, completness: 80 %, I/s(I): 3.3,
Rsym: 19.6 %, Rfree: 27.4 %
Of course, low symetry or rapid degradation in the beam is a limitation.
In this case, several crystals may be required.
Regards
JL
On 02/06/2014 11:19 AM, Joern Krausze wrote:
Dear Theresa,
We recently collected a room temperature data set from one single crystal at
Petra III. The beam line was equipped with a Pilatus detector. Data were good
to 2.7 A. In contrast, at 100 K similar crystals diffracted very poorly. So, it
is perfectly possible to obtain useful room temperature data sets from
synchrotron sources. I have to admit that in our case it certainly helped that
the crystal belonged to a high-symmetry space and full completeness was
achieved after 40 degrees angular range.
Regards,
Joern
Sent from my iPad
On 06.02.2014, at 10:51, Theresa Hsu <theresah...@live.com> wrote:
Dear crystallographers
Just out of curiosity, is it possible to collect datasets from crystals at room
temperature at synchrotron? Are fast detectors like Pilatus useful for this?
Thank you.
Theresa
--
|--------------------------------|
|Jean-Luc Ferrer |
|Institut de Biologie Structurale|
|Campus EPN |
|Synchrotron Group - Room 265 |
|6 rue Jules Horowitz |
|38027 Grenoble Cedex 1 - FRANCE |
|Ph.: +33 (0)4 57 42 85 22 |
|Fax: +33 (0)4 76 50 63 14 |
|--------------------------------|
