It¹s not just possible, but desirable! There¹s evidence that conformational ensembles can be accessed in RT crystallography data, but that these are frozen out in cryo data (aforementioned Fraser et al., Accessing protein conformational ensembles using room-temperature X-ray crystallography. PNAS, 2011, vol. 108, 16247-16252). Additionally, these ensembles have been linked to functional mechanisms (van den Bedem et al, Nat Meth (2013) 10:896-902) and to NMR order parameters (Fenwick et al, PNAS 2014 111 (4) E445-E454).
We generally see a reduction of ~0.2-0.3A in resolution from cryo to RT. And yes, Pilatus detectors help, but you can certainly get good data on other detectors. On 2/6/14, 1:51 AM, "Theresa Hsu" <[email protected]> wrote: >Dear crystallographers > >Just out of curiosity, is it possible to collect datasets from crystals >at room temperature at synchrotron? Are fast detectors like Pilatus >useful for this? > >Thank you. > >Theresa
