Lovely CCP4BB Members, Hopefully not too far off topic:
It appears that our sonicator is, ironically, bust. Whilst I wait for a new one, and/or some B-PER reagent, does anybody have a home made detergent lysis reagent (something like B-PER) recipe? Only E. coli to bust open (for now). Many Thanks Ed T.A.Edwards Ph.D. Deputy Director Astbury Centre for Structural Molecular Biology Ass. Professor, School of Molecular and Cellular Biology Garstang 8.53d University of Leeds, Leeds, LS2 9JT Telephone: 0113 343 3031 http://www.fbs.leeds.ac.uk/staff/tae/ http://www.astbury.leeds.ac.uk/people/staff/staffpage.php?StaffID=TE http://www.fbs.leeds.ac.uk/staff/profile.php?un=bmbtae -- There is something fascinating about science. One gets such wholesale returns of conjecture out of such a trifling investment of fact. ~Mark Twain
