B-PER is basically two components: 1. ~0.5% octyl-beta-thioglucopyranoside 2. 25 mM TRIS pH 7.5
A while ago when I priced out the detergent separately the bulk price came up to be about the same as the price of pre-made B-PER from Pierce. So I did not bother to check it out any further. Of course, by country it may vary significantly! Note that Y-PER is a bit more complex, its composition includes one or more polymeric detergent (like Tween, or Triton) as well as CHAPS (I never bothered to dissect its composition any further). For effective E. coli lysis over the years I converged on 3:1 mixture of BPER to YPER, supplemented with lysozyme (dirt cheap) and benzonase (homemade, via refolding from E. coli). The pH of this mixture could be adjusted fairly broadly, as well a salinity etc. but as-is the mix supports effective lysis and allows the majority of proteins I work with to stay soluble. Notably, I did find at least four proteins that did not like this lysis mixture (specifically the deterhents) - however that's 4 out of roughly 500 different proteins (I sort of stopped counting after a while). Good luck, Artem - Cosmic Cats approve of this message On Sun, Feb 9, 2014 at 10:26 AM, Thomas Edwards <t.a.edwa...@leeds.ac.uk>wrote: > Lovely CCP4BB Members, > > Hopefully not too far off topic: > > It appears that our sonicator is, ironically, bust. > > Whilst I wait for a new one, and/or some B-PER reagent, does anybody have > a home made detergent lysis reagent (something like B-PER) recipe? > Only E. coli to bust open (for now). > > Many Thanks > Ed > > > T.A.Edwards Ph.D. > Deputy Director Astbury Centre for Structural Molecular Biology > Ass. Professor, School of Molecular and Cellular Biology > Garstang 8.53d > University of Leeds, Leeds, LS2 9JT > Telephone: 0113 343 3031 > http://www.fbs.leeds.ac.uk/staff/tae/ > http://www.astbury.leeds.ac.uk/people/staff/staffpage.php?StaffID=TE > http://www.fbs.leeds.ac.uk/staff/profile.php?un=bmbtae > -- There is something fascinating about science. One gets such wholesale > returns of conjecture out of such a trifling investment of fact. ~Mark > Twain >
