Dear Blaine, The way to do this is not to downscale, but to apply a sufficiently high temperature factor. This can be done with e.g. the program CAD. However, I guess that the Bfactor will be applied to the sigma's as well, so you may want not to apply the Bfactor to the sigma's, or if this is not possible, generate a dummy sigma column and have that scaled.
Best, Herman -----Ursprüngliche Nachricht----- Von: CCP4 bulletin board [mailto:[email protected]] Im Auftrag von Mooers, Blaine H.M. (HSC) Gesendet: Donnerstag, 13. Februar 2014 15:54 An: [email protected] Betreff: [ccp4bb] create a lower resolution data set by truncating a high resolution data For some simulated phasing experiments, I want to create a lower resolution diffraction data set by truncating a high resolution data set. I would like to avoid Fourier ripples due to the truncation of the high resolution data by downscaling the data such that <I/sigma>=2.0 in the highest resolution shell of the truncated data. What is the best way to do this? Blaine Mooers Assistant Professor Department of Biochemistry and Molecular Biology University of Oklahoma Health Sciences Center S.L. Young Biomedical Research Center Rm. 466 Shipping address: 975 NE 10th Street, BRC 466 Oklahoma City, OK 73104-5419 Letter address: P.O. Box 26901, BRC 466 Oklahoma City, OK 73190 office: (405) 271-8300 lab: (405) 271-8313 fax: (405) 271-3910 e-mail: [email protected] Faculty webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/faculty/blaine-mooers-ph-d- X-ray lab webpage: http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/department-facilities/macromolecular-crystallography-laboratory Small Angle Scattering webpage: http://www.oumedicine.com/docs/default-source/ad-biochemistry-workfiles/small-angle-scattering-links.html?sfvrsn=0
