Hi, Sorry for asking an off-topic question, I have recently purified a protein having a molecular weight of 40kDa and concentration of the protein was 8mg/ml. When I tried to set the protein for crystallisation using micobatch method, the protein started precipitating in most of the buffer conditions of Crystal screen and Peg ion. The precipitation took place very quickly (within 5-10 mins). How should I overcome this problem?
Regards Prerana
