Dear Avinash,
in addition to Eleanors suggestion you might want to consider using
Dyndom in CCP4 or via its web server.
It will also identify hinge regions and percent twist motion vs. closure
motion.
Best, Matthias
-----------------------------------------
Dr. Matthias Zebisch
Division of Structural Biology,
Wellcome Trust Centre for Human Genetics,
University of Oxford,
Roosevelt Drive,
Oxford OX3 7BN, UK
Phone (+44) 1865 287549;
Fax (+44) 1865 287547
Email [email protected]
Website http://www.strubi.ox.ac.uk
-----------------------------------------
On 2/23/2014 6:57 PM, Eleanor Dodson wrote:
One way is this:
Align Domain1 of structure1 to Domain1 of structure 2 on domain 1 (using LSQKAB
or whatever.)
Then align domain2 of the output aligned structure 1 to domain 2 of structure 2
The polar Chi or Kappa or whatever output from that alignment is the angular
shift required
Eleanor
On 22 Feb 2014, at 06:19, avinash singh wrote:
Dear CCp4b users,
I have a protein which has been crystallized in two different conditions. In
one of those conditions, the structure shows the domain shifting.
Is there any programme or online server which calculates the angular shift in
domain when campared to the other condition crystallized structure which shows
no such domain shift.
Thanks in advance
Avinash Singh
