Hi SDY, It would be unlikely unless you have other reasons to believe it (biology, surrounding environment), but that blob looks a little like a possible phospho-histidine. It might be worth a looking into?
That said, buffer or metals are probably the more likely explanation. Shane Caldwell McGill University On Sat, Mar 8, 2014 at 12:28 AM, SD Y <[email protected]> wrote: > Hi, > > During refinement, I see a strong density next to His and I have tried > metal ions and water but it seems to be different. Known components of > crystallization are Tris, NaCl, BME, MgOAc, PEG3350. Its only seen in one > chain of AU. Are there any suggestions? > > Thanks in advance > SDY >
