one thing you can try and which we have used successfully (although not with an organic as the main precipitant) is too add more precipitant to the well and equilibrate for say one or two days. We often use LiCl salt as it is very soluble (you can make a 10 M solution). You can try adding to 1 M and increase if necessary. Your xtal drop should get smaller.
good luck, bert ________________________________ From: CCP4 bulletin board [[email protected]] on behalf of Yahui Liu [[email protected]] Sent: Tuesday, April 22, 2014 12:24 PM To: [email protected] Subject: [ccp4bb] Dehydration Dear all, Right now, we are try to crystallise an protein complex. By all kinds of efforts, the resolution of the crystal was about 8 angstrom. So we would like to try dehydration. The precipitant that our crystal grew was ethanol. Since the evaporation of the ethanol, our crystal is unstable at the room temperature or 4 degree when it was exposed to the air directly. Do any people have some suggestions about the dehydration way of our crystal? Best Yahui
