Hi Chris,
> In my 2.5-angstrom structure, there is negative Fo-Fc density > surrounding a metal ion after refining in Phenix. From anomalous > diffraction I am certain of the metal's identity and position in each > monomer. Also, the ion is appropriately coordinated by nearby side > chains. Should I be refining the occupancy of the ion in attempt to > "flatten" the negative density? I am considering soaking the metal ion > into crystals or cocrystallizing and collecting additional datasets. if answers to this questions found in pages 8-14 here: http://phenix-online.org/presentations/faq.pdf do not help, then please contact me directly and we will solve this problem one way or another! Pavel
