hi Jacob You'd have to ask Phil Evans for the definitive answer, but my understanding is that it's a tribute to the developers of an integration and scaling package which isn't distributed by CCP4 (and which doesn't come from the MPI either).
Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0QH Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) > On 26 Aug 2014, at 19:44, "Keller, Jacob" <[email protected]> wrote: > > I’ve chuckled at the “polish” nomenclature before, as I assumed this was a > reference to certain software developers, but if so, shouldn’t it be > “Polish?” Or does it mean polish, in the sense of shoe polish? > > JPK > > From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Harry > Powell > Sent: Tuesday, August 26, 2014 2:32 PM > To: [email protected] > Subject: Re: [ccp4bb] Problems about i > > hi > > (1) provided the predictions match the spots (i.e. indexing etc has been > successful) the best things to look at in the Integration task initially are > the graphs - if they vary reasonably smoothly, and there are no big jumps > then things have probably gone okay. > > (2) Really, the only way to reduce the mosaicity is to grow better crystals. > Are you getting much larger values than with hkl2000? How many overlaps are > you getting (both as the overall number and as a fraction of the whole?); > there may be nothing wrong at all... > > (3) For everything apart from finding the heavy atom sub-structure with > SHELXC/D/E, you want the MTZ file from ctruncate (so this is the one you want > for molecular replacement). > > For SHELXC/D/E, you really want unmerged F^2 values which can be obtained by > using the Aimless option "output polish unmerged" (as a processing option > under "Integration"). > > HTH > > Harry > -- > Dr Harry Powell, MRC Laboratory of Molecular Biology, Francis Crick Avenue, > Cambridge Biomedical Campus, Cambridge, CB2 0QH > Chairman of European Crystallographic Association SIG9 (Crystallographic > Computing) > > On 26 Aug 2014, at 16:51, 陈昂 <[email protected]> wrote: > > Dear all： > > It is my first time to use IMOSFLM instead of HKL2000. Here are my problems. > Hope you can help me. > > 1、what parameters can indicate the result quality of my imosflm? mosaicity > ,anything else? > > 2、what can I do to reduce the mosaicity and decrease bad spots,resolution or > the spot finding range? > > 3、after imosflm, I've got some MTZ maps such as original one and pointless > one,aimless one, ctruncate one. Which one should I take to the next step?what > steps do I need to take before molecular replacement and why? > > > THANKS a lot > > > Peter Chen
