Dear ravi kumar, I would not use the refined mtz-file, but the original data and run phaser with one model in fixed position, searching for a second copy.
Best, Tim On Sat, Sep 13, 2014 at 10:32:10AM +0530, Reddiravikumar Kumar wrote: > Hi, > > I am working on a protein with a resolution of 2.8 A, I have scaled it and > got solution of R-work and R-free of 0.38 and 0.43 by using 33% identical > protein as a model. My protein exits as a dimer and crystallized the dimer. > In solution I got only one molecule, i could see 80 % of sequence is > matching with electron density also Mathew's coefficient says it is two > molecules. How can I kept the previously modeled one and search for other > molecule by using the refined mtz and pdb files?. Are there any programs in > ccp4suite. please suggest me. > -- > ravi kumar -- -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A
signature.asc
Description: Digital signature
