Hi everyone, Sorry for an off-topic question.
I got a problem with crystal dissolving. Basically I got crystals of my protein in various conditions, most conditions contain PEGs but different salts. These crystals has very similar shape, so it should not be salt. Now I am trying to dissolve the crystal to make sure it is my protein, by SDS-PAGE and N-term sequencing. I washed the crystals in its original crystallization buffer few times then transfered them into regular buffer (500mM NaCl, 50mM HEPES 7.5) with or without 10mM DTT, however the crystal didn't dissolve. I then tried to heat it then add SDS loading buffer to run a gel, I did see very small amount of protein on the gel, at the correct position, but it's not enough for N-term sequencing. Is it normal for a protein crystal? And does anyone have any suggestion for dissolving such crystals?
