Vitul,

0.8 M Lithium sulphate is not excessively high salt (a 2X precipitant is possible)
You have many options available:
2M for lithium sulphate is one of them see:
http://pubs.acs.org/doi/full/10.1021/cg301531f
for various combinations of mixed cryoprotectants.

Enrico.




.On Thu, 09 Oct 2014 11:55:49 +0200, Nicolas Soler <nso...@mrc-lmb.cam.ac.uk> wrote:

Hi Vitul,

Having a final concentration of 2M for lithium sulphate worked for me
(from a 2.5M stock solution).

Nicolas

On 09/10/2014 10:27, vitul jain wrote:
Hello everybody,

can anyone please suggest a good Cryo for condition having 0.8 M
Lithium sulphate and 0.1 M  Sodium acetate 4.6.

Thanks in advance

Vitul


--
Vitul Jain
PhD student
C/O Dr. Amit Sharma
Structural and Computational Biology lab
International Center for Genetic Engineering and Biotechnology
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