Using mixed solvents is another approach:
Ciccone L., Tepshi L., Nencetti, S. & Stura E.A. (2014)
Transthyretin complexes with curcumin and bromo-estradiol: Evaluation of
solubilizing multicomponent mixtures New Biotech. 32:54–64
http://dx.doi.org/10.1016/j.nbt.2014.09.002
Volatile solvents are a problem when you try to harvest the crystals.
Enrico.
On Wed, 15 Oct 2014 20:35:33 +0200, Jurgen Bosch <jbos...@jhu.edu> wrote:
An alternative is to dissolve your compound in MeOH and dispense it
either manually or via robot, let the plate sit sometime in the hood for
faster evaporation and then add your protein + reservoir.
Jürgen
......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742<tel:%2B1-410-614-4742>
Lab: +1-410-614-4894<tel:%2B1-410-614-4894>
Fax: +1-410-955-2926<tel:%2B1-410-955-2926>
http://lupo.jhsph.edu
On Oct 15, 2014, at 5:18 PM, Keller, Jacob
<kell...@janelia.hhmi.org<mailto:kell...@janelia.hhmi.org>> wrote:
Since you mentioned EtOH, why not do this:
-Make a tray with the appropriate mother liquors
-Make a drop for each well containing mother liquor and
high-concentration ligand in EtOH (you could vary the ratios here as
needed.)
-Equilibrate by vapor diffusion until EtOH all goes into the well soln
(couple of hours at the most?)
-Add protein to these drops
You could skip right to the protein step if your protein doesn't mind
EtOH at fairly high concentrations, and anyway it will be gone fairly
quickly, esp at RT
Jacob Keller
________________________________________
From: CCP4 bulletin board
[CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>] on behalf of
Monica Mittal
[monica.mitta...@gmail.com<mailto:monica.mitta...@gmail.com>]
Sent: Wednesday, October 15, 2014 2:13 PM
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] crystallization with hydrophobic ligands
Dear All
Can anyone give suggestions for handling the solubility problem of
highly hydrophobic compounds, during co-crystallization or inhibition
assays?
The ligands I am using are almost insoluble in aquous medium. In DMSO or
95% Ethanol, the solubility is higher.
Besides crystallization, this solubility is also a hindrance for
in-vitro or in-vivo assays requiring higher conc. of ligand.
Thanks in advance !
Monica
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
http://www-dsv.cea.fr/ibitecs/simopro/ltmb/cristallogenese
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://scholar.google.com/citations?hl=en&user=Kvm06WIoPAsC&pagesize=100&sortby=pubdate
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71
