Hi Scott, it's complaining that the input files are not in the standard CCP4 map format. Since they have the extension 'pdb' and not 'map' could they by any chance be PDB format files? If so then this won't work!
Cheers -- Ian On 20 October 2014 14:47, Scott Horowitz <horow...@umich.edu> wrote: > Hi all, > > I tried using mapsig for the first time, and I got multiple errors (text > pasted at bottom). This is on mac os x 10.8.5, and the CCP4 install is > 6.4.0. I followed these instructions to be able to use CCP4 programs at the > command line: > > > http://plested.wordpress.com/2012/10/31/run-ccp4-programs-from-the-command-line-on-mac-osx/ > > http://ccp4wiki.org/~ccp4wiki/wiki/index.php?title=How_to_run_CCP4_programs_from_the_command_line > > I have also tried the csh version as well, with the same results. Any > advice would be greatly appreciated. This is my first time attempting to > use CCP4 without the GUI, so if there's additional setup necessary, that > would be great info to know. > > Thanks, > Scott > > mapsig MAPIN H96LA28I_6pt5superI.pdb MAPIN2 H96LQ17I10_6pt5superI.pdb TYPE > RATIO MAPOUT test.map > <B><FONT COLOR="#FF0000"><!--SUMMARY_BEGIN--> > <pre> > > ############################################################### > ############################################################### > ############################################################### > ### CCP4 6.4: MAPSIG version 6.4 : ## > ############################################################### > User: horowsah Run date: 20/10/2014 Run time: 09:06:01 > > > Please reference: Collaborative Computational Project, Number 4. 1994. > "The CCP4 Suite: Programs for Protein Crystallography". Acta Cryst. D50, > 760-763. > as well as any specific reference in the program write-up. > > <!--SUMMARY_END--></FONT></B> > > > ############################################# > # TRANSLATION FUNCTION MAP SIGNAL ANALYSIS. # > ############################################# > > > BE WARNED THAT THIS PROGRAM ASSUMES PERIODIC MAP BOUNDARIES, > (I.E. THE LEFT-HAND EDGE ABUTS ONTO THE RIGHT-HAND EDGE) AND > THEREFORE MAY NOT LOCATE PEAKS AT THE EDGES OF THE MAP > CORRECTLY IN OTHER CASES. IN SUCH CASES THE MAP SHOULD BE > EXPANDED IN ONE OR MORE DIRECTIONS TO MAKE IT PERIODIC IN ALL > DIRECTIONS. > <B><FONT COLOR="#FF0000"><!--SUMMARY_BEGIN--> > <html> <!-- CCP4 HTML LOGFILE --> > <hr> > <!--SUMMARY_END--></FONT></B> > >>>>>> CCP4 library signal ccp4_map:No associated header (Error) > raised in ccp4_cmap_open <<<<<< > >>>>>> CCP4 library signal ccp4_map:Cannot open file (Error) > raised in MRDHDR <<<<<< > mapsig: Error in opening input map file. > mapsig: Error in opening input map file. > Times: User: 0.0s System: 0.0s Elapsed: 0:00 > </pre> > </html> > <!--SUMMARY_END--></FONT></B> > > > -- > Scott Horowitz, Ph.D. > Research Associate > Howard Hughes Medical Institute > > University of Michigan > Department of Molecular, Cellular, and Developmental Biology > Bardwell lab > 830 N. University Ave, Room 4007 > Ann Arbor, MI 48109 > phone: 734-647-6683 > fax: 734-615-4226 > > > > -- > Scott Horowitz, Ph.D. > Research Associate > Howard Hughes Medical Institute > > University of Michigan > Department of Molecular, Cellular, and Developmental Biology > Bardwell lab > 830 N. University Ave, Room 4007 > Ann Arbor, MI 48109 > phone: 734-647-6683 > fax: 734-615-4226 >
