Hi Giulliana, I would (re)check for issues during the assignment of the space group prior to MR. Many people in the list can help you with this if you provide additional information regarding your data.
In case you have a structure that can not be solved by MR, I would give a chance for the so-called "quick cryo soaking". There are many successful histories of phasing with this approach. Some references that might be useful: http://scripts.iucr.org/cgi-bin/paper?gr0995 (*Acta Cryst.* (2000). D*56*, 232-237) http://scripts.iucr.org/cgi-bin/paper?dz5043 (*Acta Cryst.* (2005). D*61*, 1022-1030) http://www.sciencedirect.com/science/article/pii/S0959440X0200372X (Current Opinion in Structural Biology,Volume 12, Issue 5, 1 October 2002, Pages 674–678) HTH, -Alessandro [ ]s --alessandro 2014-11-05 17:20 GMT-02:00 Giulliana Rangel <[email protected]>: > Dear all, > > > I would like to known if someone could help me with some idea about the > phase problem. > > I am a beginner in crystallography and the first time I tryed to solve the > structure by Molrep, amore, mr. Bump and I didnt find anything for > molecular replacement. > > Thus, currently I've tried to soak heavy-atom ( Hg, Pt, I, Pb) and the > diffraction was good, the results in XDS didnt show so good. I didnt look > the heavy atom in density. > > I appreciate any suggestion and ideas what I could do. > > Best regards, > > > > -- > Giulliana Rangel > Mestranda PPG-Biotecnologia UNIFESP > Laboratório de Biologia Estrutural > Tel.: (12) 3309-9698 > Rua Talim 330, Vila Nair > CEP 12231-280 > São José dos Campos - SP > >
