Well I think some companies make the screens from stocks, the others adjust the pH of the final mixture, so you might want to check the small print for the screen you used in the first place…
But when optimizing with your own solutions, you would in general take the easy way out - make good stocks and mix them as you wish. Petri Petri Kursula, PhD ---------- Professor of Biochemistry and Molecular Biology Department of Biomedicine University of Bergen, Norway http://www.uib.no/en/persons/Petri.Kursula <http://www.uib.no/en/persons/Petri.Kursula> [email protected] <mailto:[email protected]> ---------- Project Leader, Docent Faculty of Biochemistry and Molecular Medicine Biocenter Oulu University of Oulu, Finland [email protected] <mailto:[email protected]> ---------- > On 21 Nov 2014, at 17:48, amro selem > <[email protected]> wrote: > > Dear All, > first i wish you nice weekend, then i wanna ask about formulation of my own > mother liquor, i want to optimize a condition containing > 0.2m MgCl2, 0.1m Tris , PH 8; and 20% PEG6K as pricipitant. (PACT) > the question is , should i make a stock solution from every ingredient by > dissolving it in miliQ water. then mix all stuff together and add water > till final concentration with out adjusting final pH. OR dissolve the PEG in > pH adjusted buffer and then mix all ingredients , so the final pH will be 8. > any suggest any idea or any one knows what the company do. > CHEERS > Amr >
