Dear all,
I ran into a problem when I was solving a structure using SAD. After
determining phase and building an initial model using AutoSol, I suddenly
found that every two well ordered protein molecule layers were separated by
a large empty space that extended across the entire crystal. The void space
is so large that another two layers of molecules could be fit in. In that
case, the unit cell was extremely extended across the empty space and the
solvent content was very high. How does a crystal pack like that? or did I
do something wrong during phasing to make such kind of artifact?
Best,
David
