Hi, I'm inclined to say that you may have indexed your data with the wrong space group -your description violates the definition of a crystal. What happens if you index in P1 and resolve the x-tal using the structure from your current phases as an MR search model? This is assuming that you have reasonable phases to build a reasonable model. Best wishes, Reza
Reza Khayat, PhD Assistant Professor The City College of New York Department of Chemistry, MR-1135 160 Convent Avenue New York, NY 10031 Tel. (212) 650-6070 www.khayatlab.org ---- Original message ---- >Date: Thu, 27 Nov 2014 13:33:26 -0500 >From: CCP4 bulletin board <[email protected]> (on behalf of kuan hu <[email protected]>) >Subject: [ccp4bb] large empty spaces in solved crystal structure >To: [email protected] > > Dear all, > > ******** I ran into a problem when I was solving a > structure using SAD. After determining phase and > building an initial model using AutoSol, I suddenly > found that every two well ordered protein molecule > layers were separated by a large empty space that > extended across the entire crystal. The void space > is so large that another two layers of molecules > could be fit in. In that case, the unit cell was > extremely extended across the empty space and the > solvent content was very high. How does a crystal > pack like that? or did I do something wrong during > phasing to make such kind of artifact? > > Best, > > David
