You can try adding 10% or 20% glycerol to your Cryo that works for us. -------------------------------------------- On Tue, 23/12/14, Yarden Opatowsky <[email protected]> wrote:
Subject: Re: [ccp4bb] best cryprotectant To: [email protected] Date: Tuesday, 23 December, 2014, 2:47 PM Adding Tacsimate on top of the basic conditions worked best for us. On Tue, Dec 23, 2014 at 11:11 AM, rohit kumar <[email protected]> wrote: Dear all, I got crystals in 1.4 M sodium/potassium phosphate, ph-8.2 condition. Could anyone tell me what is the best cryprotectant for this above condition. Thanks -- WITH REGARDS Rohit Kumar Singh Lab. no. 430, P.I. Dr. S. Gourinath, School of Life Sciences, Jawaharlal Nehru University New Delhi -110067 -- Dr. Yarden Opatowsky The Mina & Everard Goodman Faculty of Life Sciences Bar-Ilan University Ramat Gan 52900 Israel email: [email protected] tel : 972-3-5318330 fax: 972-3-7384058
