We usually try 25-30% glucose first. Weigh out 150 mg of glucose in a
microcentrifuge tube add well solution, dissolve, mix, and make up to
the 0.5 mL mark with well solution. If crystals crack in this solution,
make up a 40% glucose solution in mother liquor, and add this solution
in 0.25 drop volume increments to your crystallization drop over 5-10
minutes until you have added 4 drop volumes.
Cheers,
_______________________________________
Roger S. Rowlett
Gordon & Dorothy Kline Professor
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: [email protected]
On 12/23/2014 4:11 AM, rohit kumar wrote:
Dear all,
I got crystals in 1.4 M sodium/potassium phosphate, ph-8.2 condition.
Could anyone tell me what is the best cryprotectant for this above
condition.
Thanks
--
WITH REGARDS
Rohit Kumar Singh
Lab. no. 430,
P.I. Dr. S. Gourinath,
School of Life Sciences,
Jawaharlal Nehru University
New Delhi -110067
