Hi, Warmed up capillary and hamilton syringe works fine (i used heat lamps similar to pizza heaters used by all-night pizza joints). You can get a cheap heat lamp from most pet stores (terrarium heaters).
Artem On Jan 5, 2015 11:32 AM, "Georg Mlynek" <[email protected]> wrote: > Dear Colleagues, > > After reading a few papers about growing suitable crystals for neutron > diffraction. I will do capillary counterdiffusion with an agarose plug > between mother liquor and protein solution like described in > http://www.ncbi.nlm.nih.gov/pubmed/23192028. > > However I looked quite some time now, to find how to put the 100 ul 1% > low-melting agarose plug in the middle of the capillary, but was not > successful. Does one use a gel loading tip or is there a better way to do > this? > > Thanks in advance for any tips and tricks, > > best regards, Georg. >
