Dear Georg, There are different ways to set-up counterdiffusion experiment but the set-up you mentioned is done in a single capillary. The easiest way is to prepare the agarose and when it is still warm pipette the 100 microL on any surface, I use a piece of parafilm. Them you have to adsorb it with the help of a silicon-tube or syringe connected to one end of the capillary (see Fig. 1b of Acta Cryst. (2010). F66, 264–268). Place the small column of agarose at the center of the capillary and allow it gel. You can place the capillaries in the fridge to speed it up. When the agarose has gel you may add the protein and precipitant solution each one to one end of the capillaries using Hamilton syringes or capillaries of small diameter. Finally seal both ends with either bee-wax or vacuum grease.
Cheers Gavi. 2015-01-05 18:25 GMT+01:00 Georg Mlynek <[email protected]>: > Dear Colleagues, > > After reading a few papers about growing suitable crystals for neutron > diffraction. I will do capillary counterdiffusion with an agarose plug > between mother liquor and protein solution like described in > http://www.ncbi.nlm.nih.gov/pubmed/23192028. > > However I looked quite some time now, to find how to put the 100 ul 1% > low-melting agarose plug in the middle of the capillary, but was not > successful. Does one use a gel loading tip or is there a better way to do > this? > > Thanks in advance for any tips and tricks, > > best regards, Georg. > -- ____________________________________ Dr. José A. Gavira Gallardo Laboratorio de Estudios Cristalográficos IACT, (CSIC-UGR) Av. de las Palmeras, 4 18100 Armilla (Granada) Tel.: 958 230000 Ext.190205 Fax: 958 55 26 20 e-mail: [email protected] web: http://www.lec.csic.es/gavi ____________________________________
