The Bead Beater has a 15, 40, and 350 mL chambers. I haven't used mine
to homogenize yeast, but I suspect it is similar in performance to E.
coli disruption. (Different bead sizes are used for yeast than
bacteria.) We get excellent, gentle disruption of E. coli in 8 minutes
total. A French Press takes a loooooooooong time for more than 40-50 mL
of lysate, and often requires multiple passes for complete disruption.
Beads and cell wall debris can be easily removed by centrifugation for
direct application on your FPLC.
We moved to Bead Beaters from a French Press more than a decade ago and
haven't looked back. We can do one liter overexpression preps (20-30 g
wet packed cells) in the 40 mL chamber.
Cheers,
_______________________________________
Roger S. Rowlett
Gordon & Dorothy Kline Professor
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: [email protected]
On 1/12/2015 10:44 AM, Johnson Luwang wrote:
Dear All,
I am looking for an equipment that can do /Saccharomyces
cerevisiae/ cells lysis (say about 100-200ml lysate volume). I have
used the Constant Systems cell disruptor TS 0.75kW model (at another
facility) that can go up to 40kpsi. It works perfect for my yeast
cell lysis experiment. We were planning to set up similar facility
at our place, but some of my colleagues suggested me about "bead
beater" for the same purpose. Quite frankly I don't have much idea
about bead beater. And I need the suggestions from the experts who
have used these two systems (french press and bead beater). Is the
bead beater better than the french press for yeast cell lysis? If so
can you suggest me a bead beater model which is the best for our purpose?
Many thanks.
Best Regards,
Johnson L.W.
