We are huge fans of the Retsch Planetary Ball Mill grinder. Grinds yeast cells (different volumes) at liquid N2 temp. Minimal proteolysis will high efficiency lysis. Made an enormous difference to obtaining a higher yield of intact, functional protein complexes.
-Mary Munson UMass Medical School From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Johnson Luwang Sent: Monday, January 12, 2015 10:45 AM To: [email protected] Subject: [ccp4bb] Queries regarding bead beater and french press. Dear All, I am looking for an equipment that can do Saccharomyces cerevisiae cells lysis (say about 100-200ml lysate volume). I have used the Constant Systems cell disruptor TS 0.75kW model (at another facility) that can go up to 40kpsi. It works perfect for my yeast cell lysis experiment. We were planning to set up similar facility at our place, but some of my colleagues suggested me about "bead beater" for the same purpose. Quite frankly I don't have much idea about bead beater. And I need the suggestions from the experts who have used these two systems (french press and bead beater). Is the bead beater better than the french press for yeast cell lysis? If so can you suggest me a bead beater model which is the best for our purpose? Many thanks. Best Regards, Johnson L.W.
