Hi Fred,
2015-02-06 11:58 GMT+01:00 Kerff Fred <[email protected]>: > Hello, > > Looking at structure 2HR0 ("The structure of complement C3b provides > insights into complement activation and regulation. »,Abdul Ajees, A., > Gunasekaran, K., Volanakis, J.E., Narayana, S.V., Kotwal, G.J., Krishna > Murthy, H.M.; (2006) Nature 444: 221-225), I noticed the absence of > contacts between layers in the crystal. Is it something that has already > been observed in other crystals? > > Come to think of it, in the communication ( http://www.nature.com/nature/journal/v448/n7154/full/nature06103.html) Ajees et al., mention that they actually don't have gaps, but just some low occupancy contaminant that makes crystal contacts. So, even with this structure being fraud, you would not be able to use it as an example of absence of crystal contacts. Best regards, Folmer Fredslund > Best regards, > > Fred > ----- > Frédéric Kerff > Chercheur qualifié F.R.S.-FNRS > Cristallographie des protéines > Centre d'Ingénierie des Protéines > Université de Liège > 17, Allée du 6 Août - Bat B5a > 4000 Liège (Belgium) > Tel.: +32 (0)4 3663620 > Fax: +32 (0)4 3663772 > > > > > Le 6 févr. 2015 à 10:12, Tim Gruene <[email protected]> a écrit : > > > > -----BEGIN PGP SIGNED MESSAGE----- > > Hash: SHA1 > > > > Dear Smith, > > > > The sca file most likely does not contain flags. pointless can read > > the sca file, standardise it to ccp4 standards and freerflag marks > > random reflections. You should use the maximum of 500 unique > > reflections or 5% of the unique reflections, whichever is larger. > > > > Best, > > Tim > > > > On 02/06/2015 09:49 AM, Smith Lee wrote: > >> Dear All, I have a sca file. Will you please tell me by which > >> software or how I can know whether the sca file contains R-free > >> tags? If not, by which software or how I can add the R-free tags? > >> And how much of the reflections I add the R-free tags? I am looking > >> forward to getting your reply. Smith > >> > > > > - -- > > - -- > > Dr Tim Gruene > > Institut fuer anorganische Chemie > > Tammannstr. 4 > > D-37077 Goettingen > > > > GPG Key ID = A46BEE1A > > > > -----BEGIN PGP SIGNATURE----- > > Version: GnuPG v1.4.12 (GNU/Linux) > > > > iD8DBQFU1IWVUxlJ7aRr7hoRAmZHAJ4+6wREnwkFN0EhfErAA0tPSopKKwCgiLdi > > j0JFZac4kAh8twpov71MG84= > > =XN57 > > -----END PGP SIGNATURE----- > -- Folmer Fredslund
