Bringing the pH closer to the measured pI would definitely help. The pI can be measured on an IEF gel. Glycosylation, if applicable, could dramatically increase solubility.
Roger Rowlett On Feb 16, 2015 11:33 PM, "Mattiroli,Francesca" < [email protected]> wrote: > Hi all, > > I am struggling with a protein complex that is too soluble. I have reached > about 20 mg/ml but I still observe very little precipitation (clear drops > in 90-95% of the tested conditions). The proteins are expressed in insect > cells and going to higher concentration is not easily achievable. > I have tried different buffer conditions (salt concentration and pH) and I > am testing temperatures. I am at a loss with what to try next. > Do you think PTMs (phosphorylation, acetylation) might be causing this? > Any input on how to decrease solubility? > > Thank you very much in advance, > > Francesca > > >
