Hi Francesca, Try some zinc (1mM + ) in your protein buffer? Zinc tends to make a lot of things less soluble in my hands.
Dave On Tue, 17 Feb 2015 04:34 Mattiroli,Francesca < [email protected]> wrote: > Hi all, > > I am struggling with a protein complex that is too soluble. I have reached > about 20 mg/ml but I still observe very little precipitation (clear drops > in 90-95% of the tested conditions). The proteins are expressed in insect > cells and going to higher concentration is not easily achievable. > I have tried different buffer conditions (salt concentration and pH) and I > am testing temperatures. I am at a loss with what to try next. > Do you think PTMs (phosphorylation, acetylation) might be causing this? > Any input on how to decrease solubility? > > Thank you very much in advance, > > Francesca > > >
