a horrible water molecule would fit in there :-)
On 23/02/15 19:42, Keller, Jacob wrote:
A little more info:
-Resolution is 1.75 Ang at edge of detector, but I/sig was still ~3; pushing
integration to corners gives 1.66 Ang, but completeness is horrible. The home
setup was at the limit (without 2 theta.)
-Data are horribly twinned with fractions of 58/42%, spacegroup is P3212.
Besides that, though, the data are really excellent.
-Peak is 6.40 rmsd in Refmac-generated difference map.
-Distance from blob-peak to Arg-N is 3.43 Ang, but the peak is obviously not
spherical.
JPK
-----Original Message-----
From: Keller, Jacob
Sent: Monday, February 23, 2015 12:34 PM
To: Keller, Jacob; CCP4BB@JISCMAIL.AC.UK
Subject: RE: Blob Game
...And the cryoprotectant was simply the Li/NH4SO4 mother liquor. Also maybe
relevant: the crystals were pretty old, a year or so.
JPK
-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Keller,
Jacob
Sent: Monday, February 23, 2015 12:21 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Blob Game
Dear Crystallographers,
I've got a strange blob hanging off an arginine--see attached. Any ideas?
Nothing weird in the prep; cryst+prot conditions: NH4SO4, TRIS/HEPES, CaCl2,
NaCl, Li2SO4. There are really no other side chains which could be close
enough, and there's nothing in the anomalous LLG map.
???
JPK
*******************************************
Jacob Pearson Keller, PhD
Looger Lab/HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
email: kell...@janelia.hhmi.org
*******************************************
--
J. Preben Morth, Ph.D
Group Leader
Membrane Transport Group
Nordic EMBL Partnership
Centre for Molecular Medicine Norway (NCMM)
University of Oslo
P.O.Box 1137 Blindern
0318 Oslo, Norway
Tel: +47 2284 0794
