Just a little mother liquor. I am seeing what SO4 does in refinement right now.
JPK -----Original Message----- From: Boaz Shaanan [mailto:[email protected]] Sent: Monday, February 23, 2015 2:50 PM To: Keller, Jacob; [email protected] Subject: RE: [ccp4bb] Blob Game Boaz Shaanan, Ph.D. Dept. of Life Sciences Ben-Gurion University of the Negev Beer-Sheva 84105 Israel E-mail: [email protected] Phone: 972-8-647-2220 Skype: boaz.shaanan Fax: 972-8-647-2992 or 972-8-646-1710 ________________________________________ From: CCP4 bulletin board [[email protected]] on behalf of Keller, Jacob [[email protected]] Sent: Monday, February 23, 2015 8:53 PM To: [email protected] Subject: Re: [ccp4bb] Blob Game >Ethanol? You weren't setting up drops with liquor on your breath, were >you I can't remember. Ahah, so you did have some liquor. Good. Seriously, if there is something below the blob in the direction that you sent us and could be joined with the peak, couldn't an SO-4 fit in there somehow? It's too detailed for water at this resolution, isn't it? Boaz JPK On 02/23/2015 12:20 PM, Keller, Jacob wrote: > Dear Crystallographers, > > I've got a strange blob hanging off an arginine--see attached. Any ideas? > Nothing weird in the prep; cryst+prot conditions: NH4SO4, TRIS/HEPES, CaCl2, > NaCl, Li2SO4. There are really no other side chains which could be close > enough, and there's nothing in the anomalous LLG map. > > ??? > > JPK > > ******************************************* > Jacob Pearson Keller, PhD > Looger Lab/HHMI Janelia Research Campus > 19700 Helix Dr, Ashburn, VA 20147 > email: [email protected] > ******************************************* > >
