Hi Stephen,
we crystallized several proteins in a glove box placed in a room with
air condition. We checked the temperature all the times and found that
is had been quite stable.
For one protein that crystallized only at 4 deg C, we setup the
crystallization plate within the glove box and put it into an gas-tight
plexiglass box (built by our workshop). The box with the crystallization
plate was then incubated at 4degC.
Best, Guenter
Dear CCP4BBer's
Apologies for the off-topic post, but the CCP4BB seems to be the best place to
ask about crystallisation.
I am looking to set up crystallisation in an anaerobic glove box and wondered
how other people did this, specifically the crystallisation stage. My initial
thoughts were to place a small crystallisation incubator inside the box,
however the smallest I have come across so far (~27L) is still rather large.
Has anyone come across smaller incubators? Alternatively are incubators even
neccessary if the glove box is placed in a room with good air conditioning and
stable temperature control?
Any recommendations would be very helpful.
Thanks in advance,
Steve Carr
Dr Stephen Carr
Research Complex at Harwell (RCaH)
Rutherford Appleton Laboratory
Harwell Oxford
Didcot
Oxon OX11 0FA
United Kingdom
Email stephen.c...@rc-harwell.ac.uk
tel 01235 567717
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