I also wondered about the statement about oils blocking diffusion of O2. We
had lots of trouble keeping things anaerobic in a glove box until we degassed
the oils and waxes used to mount crystals in capillaries. We found that
putting them under vacuum removed much of the dissolved oxygen. The waxes
required cycling between heating and vacuum several times. Ron
On Wed, 18 Mar 2015, Edward A. Berry wrote:
Do you have evidence that the oil blocks diffusion of O2? O2 is a nonpolar
molecule, generally much more soluble in oils than in water. I'm not sure
about silicone oils, but I would think they also dissolve O2 readily.
eab
On 03/18/2015 08:02 AM, Patrick Shaw Stewart wrote:
Hi Steve
I have one more comment for this thread.
The microbatch-under-oil method is very handy for anaerobic work:
1. You can keep the microbatch stock solutions in normal microtitre
plates (polypropylene is best to reduce evaporation) for months, which
hugely reduces the amount of degassing that you need to do. You will only
use say 0.5 ul of stock per drop.
2. The oil offers a surprising amount of protection from oxidation,
which may be helpful eg in harvesting.
3. Microbatch can be automated - in parallel to vapor diffusion if
desired
It's amazing how often (aerobic) microbatch produces far superior crystals
to V.D. for no obvious reason - it's well worth trying for both screening
and optimization.
Best wishes
Patrick
On 11 March 2015 at 10:17, <Stephen Carr> <stephen.c...@rc-harwell.ac.uk
<mailto:stephen.c...@rc-harwell.ac.uk>> wrote:
Dear CCP4BBer's
Apologies for the off-topic post, but the CCP4BB seems to be the best
place to ask about crystallisation.
I am looking to set up crystallisation in an anaerobic glove box and
wondered how other people did this, specifically the crystallisation stage.
My initial thoughts were to place a small crystallisation incubator inside
the box, however the smallest I have come across so far (~27L) is still
rather large. Has anyone come across smaller incubators? Alternatively
are incubators even neccessary if the glove box is placed in a room with
good air conditioning and stable temperature control?
Any recommendations would be very helpful.
Thanks in advance,
Steve Carr
Dr Stephen Carr
Research Complex at Harwell (RCaH)
Rutherford Appleton Laboratory
Harwell Oxford
Didcot
Oxon OX11 0FA
United Kingdom
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