Dear All, This is regarding the protein structure model building using the density files or the .mrc file obtained by cryo-electron microscopy data. I want to seek some help on how to visualize these files in COOT. I am working on mac system and have recently installed CCP4 which has Coot- Dec 2014 version. When I load mrc file in coot via open map it gives error: map failed to open. Then using the em2em program, i have been successful in converting it to .ccp4 which I am able to open it in the coot. Please let me know if this the way I am supposed to do it.
I have one more query that may be very naive but at which sigma level one should work towards these maps in coot ? I have read the paper by Brown et al, in ACTA D, 2015, they have mentioned the tools for macromolecular model building and refinement into electron cryo-microscopy and mentioned many useful tools in COOT like jiggle fit etc for this. So, if I can use ccp4 also in the same way like we do for crystallography and do refinement after model building in coot.For information, the protein I am working on has around 8A resolution. Looking forward for the help and guidance. Regards
