Hi Jürgen, sorry - that's what I get when mailing while boarding ... No, I'd just collect 360 degrees, and if the crystal is still ok, another 360, ... This way one - obtains high completeness and multiplicity - can discard frames with "too much" radiation damage - does not have to worry about the starting point of data collection. To make the most of the second 360°, you should change some parameter: wavelength, rotation axis (requires a BL with kappa or Prigo), or at least distance (by few percent).
When I read that 1° frames are collected, I just wonder why? Because it used to be done like that in the good old times? HTH, Kay On Fri, 17 Apr 2015 11:55:42 +0000, Jurgen Bosch <[email protected]> wrote: >Just to clarify, I think what Kay meant with "strategy" is that you don't just >shoot at the crystal and collect. You should figure out what is the optimum >start and end point of your data collection. Best to be cautious and not >immediately go for highest resolution and not fry your crystal. A 4 A complete >anomalous data set is better than a partial 3.2A one. >J?rgen > > >...................... >J?rgen Bosch >Johns Hopkins University >Bloomberg School of Public Health >Department of Biochemistry & Molecular Biology >Johns Hopkins Malaria Research Institute >615 North Wolfe Street<x-apple-data-detectors://4>, W8708 >Baltimore, MD 21205<x-apple-data-detectors://5/0> >Office: +1-410-614-4742<tel:%2B1-410-614-4742> >Lab: +1-410-614-4894<tel:%2B1-410-614-4894> >Fax: +1-410-955-2926<tel:%2B1-410-955-2926> >http://lupo.jhsph.edu<http://lupo.jhsph.edu/> > >On Apr 17, 2015, at 06:37, Kay Diederichs ><[email protected]<mailto:[email protected]>> wrote: > >Hi, >I'd say using a Pilatus detector in fine-slicing mode and lowdose/high >multiplicity will give you better chances to solve the structure. The right >strategy makes a difference ... >Best, >Kay >
