How long are you cryoprotecting for? You don't really need more than a few seconds. If I have sensitive crystals i tend to just "swipe" them slowly through the final CP solution and often that works. If I would leave them in the CP solution for more than say 30 seconds the crystals would behave like you describe.
GL Bert ________________________________________ From: CCP4 bulletin board [[email protected]] on behalf of Faisal Tarique [[email protected]] Sent: Monday, May 04, 2015 7:43 PM To: [email protected] Subject: [ccp4bb] cryo condition Hello everyone Can anybody suggest me a cryo condition for a crystal obtained in MIDAS screen of Molecular Dimension: G1> 0.1MTris8.0G10.1Mpotassium chloride25% v/vSOKALAN®CP7 0.1MHEPES7.0 G2>0.3Mammonium formate20% v/vSOKALAN® CP 5 0.1MHEPES7.0 Crystals are in beautiful cuboid shaped but all sorts of PEG combinations and Glycerol formulation failed to prevent it from cracking and dissolving. Has anybody faced a similar situation as mentioned above and what precaution was taken to prevent it from cracking or dissolving. Your suggestions will be of immense help Thanks in advance -- Regards Faisal School of Life Sciences JNU
