Magnesium phosphate crystals? I've definitely seen salt crystals with
that morphology before. Check out the Ksp for Mg3(PO4)2.
Engin
On 5/17/15 6:45 PM, Bishwa Subedi wrote:
Hi All,
I know that its hard to say if the crystal is protein crystal just by looking
at its morphology, however I was wondering if any of you have ever come across
protein crystals as one attached here. It would help build up my confidence :)
and work further until I put them on to X-ray beam. I could notice similar
crystals with two different proteins one in the initial screen and one after pH
screening the similar condition and involves NPS (Nitrate, phosphate and
Sulphate) from Morpheus screen. To mention, MgCl2 (0.01M) is also added
directly to the protein as a co-factor.
Crystal condition : NPS (0.9M)+ Buffer System 2 0.1M (i.e. HEPES and MOPS) as
well as with buffer system 3 0.1M (TRIS and BICINE) at pH 7.5 and 8.5
respectively and P550MME_P20K 30.0%
Thank you for your opinion.
Bishwa
