Dear Bishwa,What about Hamptons Izit dye.
S.M.Jaimohan, Ph.D
On Monday, 18 May 2015 7:50 AM, Engin Özkan <[email protected]> wrote:
Magnesium phosphate crystals? I've definitely seen salt crystals with
that morphology before. Check out the Ksp for Mg3(PO4)2.
Engin
On 5/17/15 6:45 PM, Bishwa Subedi wrote:
> Hi All,
>
> I know that its hard to say if the crystal is protein crystal just by looking
> at its morphology, however I was wondering if any of you have ever come
> across protein crystals as one attached here. It would help build up my
> confidence :) and work further until I put them on to X-ray beam. I could
> notice similar crystals with two different proteins one in the initial screen
> and one after pH screening the similar condition and involves NPS (Nitrate,
> phosphate and Sulphate) from Morpheus screen. To mention, MgCl2 (0.01M) is
> also added directly to the protein as a co-factor.
>
> Crystal condition : NPS (0.9M)+ Buffer System 2 0.1M (i.e. HEPES and MOPS)
> as well as with buffer system 3 0.1M (TRIS and BICINE) at pH 7.5 and 8.5
> respectively and P550MME_P20K 30.0%
>
> Thank you for your opinion.
>
> Bishwa
