Hi Giulliana, here is a nice and simple method on how to select heavy atoms for phasing using native PAGE (please see Fig. 1): http://www.ncbi.nlm.nih.gov/pubmed/10903954 Structure. 2000 Jul 15;8(7):R143-9. Screening for phasing atoms in protein crystallography. Boggon TJ, Shapiro L. Hope that helps, Tomas
On Mon, Jun 29, 2015 at 8:47 AM, Giulliana Rangel <[email protected]> wrote: > Dear all, > > I'm looking for a method to solve the phase problem. Thus, I would like some > help about heavy-atom derivatives. > > What is the best heavy-atom to make soaking? I tried Iodine (because my > protein have a lot of tyrosines), but no results... > > I read some papers about quick-soak, its a good method? Using platine, Hg, > Br... > > Any suggestions? Thank you so much. > > Best wishes, > > -- > Giulliana Rangel > M.S. Biotechnology Program - UNIFESP > Structural Biology Laboratory > Phone: +55 (12) 3309-9698 > 330 Talim St > ZIP CODE 12231-280 > São José dos Campos - SP/ BR >
