Hi everyone, I think I have finally got a solution in Phaser (screen shot attached) as the TFZ is 10. However the solution PDB has 30 molecules in it as the search model was an NMR solution. As I have 0.944 Angs resolution data pretty complete, I thought of building the initial model in ArpWarp. I prepared a new PDB file form the Phaser solution output with only one molecule instead of 30. Prepared the sequence file in pir format for the target. Now the wilson plot says B-factor of 3.66 and solvent content 0.98 even if I cut off the low resolution data to 8.0 from 20.0 and increase the high resolution to 1.0. Initially when I calculated the mathews coefficient it showed the best estimate to be 2 molecules in the assymmetric unit. Am I missing something again?
Thanks in advance, Ivan
