From your picture it looks like you have both these needle clusters and single 
needle/rod-lke crystals. The diffraction pattern you show is what you expect 
from a needle cluster (with the ring-like patterns at low resolution), a single 
needle or a larger optimized single crystal should give you no trouble. 


Petri Kursula, PhD
Professor of Biochemistry and Molecular Biology
Department of Biomedicine
University of Bergen, Norway 
<> <>
Project Leader, Docent
Faculty of Biochemistry and Molecular Medicine
Biocenter Oulu
University of Oulu, Finland <>

> On 12 Oct 2016, at 08:54, Liu Rachel <> wrote:
> Dear everyone:
> Recently, I suffered a problem during my research work. I purified a zinc 
> finger protein, and crystallized as a beautiful cube in a reservoir solution 
> only containing phosphate as the precipitant, no other buffer or molecules. 
> However, regardless of multiple optimization, the crystal diffracted badly 
> (7~8 Å best). I have also tried co-crystallization with dsRNA because this 
> protein can  bind to dsRNA. Then crystals grow in a new condition(2.5M 
> (NH4)2SO4,0.1M BTP,  pH7.0)and its form change to cluster of needle. But the 
> X-ray diffraction diagram is very strange(as shown in the picture). The Data 
> cannot be processed with HKL2000 either. I want to figure out, could this be 
> a RNA crystal rather than the complex?   Or is there anybody know about the 
> crystal of RNA molecular?
> Thank you very much!
> Yujie Liu 
> Room 2071, research center in life sciences,
> China Agricultural University 
> No. 2 yuanmingyuan west road, Haidian District, Beijing, 100193  P.R. China 
> Tel: (86)-10-62734078
> <crystal.jpg><lyj.png>

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