Hi Lingyuan, 1) you're very lucky (but you already know that) 2) crystals grown quickly in bulk rarely diffract well. I would follow up by a conventional droplet crystallization against a concentrating agent - in the cold - such that your protein gradually concentrates from [X] to 2-3 times [X] and that should presumably give you crystals. Another option is temperature-driven crystallization (if this is what is happening) in which case a simple setup consists of a sitting or hanging drop plate inside a closed styrofoam box that you set up at room temperature, then bring the box (already closed) into a cold room - the box adds a considerable delay factor. If you want to extend the cooling process even more you could place a few Blue Ice packs in the box (again, at room temperature).
Artem www.harkerbio.com "protein crystals for breakfast, lunch, AND dinner" - Cosmic Cats approve of this message On Tue, Oct 18, 2016 at 6:41 AM, kiki <kiki-onl...@qq.com> wrote: > Dear all users, > > I got my crystals at the bottom of the tube on ice just before I started > to screen crystals. Those crystals' shapes were good but easy to melt when > I harvested them. I shot these crystals. They only diffracted to 7A to 8A. > Has anyone ever met this situation before? How to improve? > > Thanks, > Lingyuan >
