Good morning,

53 x 2 = 106
79 x 2 = 158

Awfully close to 156 and 105 in the C-group. Are you sure there is no
tricky twinning or faulty indexing of some sort - in either of these space
group assignments?

On the other hand, I have seen two different habits and different
spacegroups growing from the same drop. So maybe you are just lucky?

Artem
www.xtals.org
'crystals the size of cats, *every time*'


- Cosmic Cats approve of this message

On Fri, Oct 28, 2016 at 9:13 AM, Sam Tang <[email protected]> wrote:

> Dear all
>
> Sorry for going a bit off-topic in this thread.
> May I seek your advice as on whether you have experienced that crystals
> being obtained from the same droplet, looking alike under microscope (rod
> shape) and in fact growing possibly from a same nuclei, give two space
> groups after indexing?
>
> I recently obtain crystals for a protein (co-crystallized with a nucleic
> acid ligand) and collected two datasets from synchrotron. Although these
> two crystals are from the same drop, the SG and unit cell dimensions are
> very different:
>
> Xtal1: C121 (156 60 105 90 111 90) (L-test, Pointless shows that there is
> no twinning), ~2.5 Angstrom
> Xtal2: P1 (53 60 79 106 105 98), ~3 Angstorm
>
> Would it be possible that the ligand changes the SG of the crystal so that
> only one of the forms contains the ligand?
>
> Any advice is appreciated and thanks a lot in advance for your input.
>
> Regards
>
> Sam Tang
> Biochemistry Programme, School of Life Sciences, CUHK
>
>

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