Dear all, for reasons difficult to explain in a brief email, I am interested in flipping the order of domains in a poli-peptide in bacteria. The scenario would be a multi-domain protein, let's say containing 3 domains DI, DII and DIII, which are arranged N-ter to C-ter: DI-DII-DIII. The protein is expressed at reasonable, soluble levels in E.coli. Given that nowadays it is easy and cheap to order synthetic genes, I am planing to re-arrange the sequence of domains from DI-DII-DIII to DIII-DII-DI. Does anyone envision any mayor problem with such a crazy idea? Any pointers to previous literature on the issue?
Thanks so much for your time and best wishes. -- Israel S. Fernandez PhD
