Impossible to say without knowing details of the particular protein. Could be trivial or could be impossible.
Artem www.harkerbio.com "See us at PSDI 2016 in Malmo" (some messages are just what they seem) On Nov 13, 2016 1:04 AM, "Israel Sanchez" <[email protected]> wrote: > Dear all, > > for reasons difficult to explain in a brief email, I am interested in > flipping the order of domains in a poli-peptide in bacteria. The scenario > would be a multi-domain protein, let's say containing 3 domains DI, DII and > DIII, which are arranged N-ter to C-ter: DI-DII-DIII. The protein is > expressed at reasonable, soluble levels in E.coli. Given that nowadays it > is easy and cheap to order synthetic genes, I am planing to re-arrange the > sequence of domains from DI-DII-DIII to DIII-DII-DI. Does anyone envision > any mayor problem with such a crazy idea? Any pointers to previous > literature on the issue? > > Thanks so much for your time and best wishes. > > -- > Israel S. Fernandez PhD > > >
