What is the resolution? Breaks can be caused by anisotropic data - but that map does not look right. Is that picture taken from COOT ?
Eleanor On 1 December 2016 at 14:37, Reza Khayat <[email protected]> wrote: > I would have to agree with Tim. I'm a little surprised that your R/Rf is > 27/32. Are the images representative of the entire model/map fits, or a > small section of it? > > Best wishes, > Reza > > Reza Khayat, PhD > Assistant Professor > Department of Chemistry > City College of New York > 85 Saint Nicholas Terrace, CDI 2.318 > New York, NY 10031 > http://www.khayatlab.org/ > 212-650-6070 > > -----Original Message----- > From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Tim > Gruene > Sent: Thursday, December 01, 2016 9:19 AM > To: [email protected] > Subject: Re: [ccp4bb] visible side-chains but breaks in main-chain > > Dear V, > > your map still looks quite poor and disconnected. Maybe you could improve > your overall model before you worry about such detailed questions like main > chain vs. side chain density. > > If your resolution is not too poor, you could try rebuilding your model > with shelxe, or buccanner, or phenix, or ... > > Regards, > Tim > > On Thursday, December 01, 2016 02:35:21 PM Veronica Fiorentino wrote: > > Dear bb-ers, > > Is it uncommon to see side-chains for PHE/ARG visible in density but > > the main-chain density breaking (say within a beta-strand)? I have > > attached 1.FEM (map) 2&3. Experimental phase map. If I refine the > > model as Poly-ala, the side-chains appear green in difference map. I > > therefore assume that my sequence assignments are correct(?). Is this > > symptomatic of some big mistake? > > > > R/Rfree are around 27/32 % respectively. The phase problem was solved > > by SeMet phasing. > > > > Many thanks > > V[image: Inline images 1] > > > > [image: Inline images 2] > > > > [image: Inline images 3] > -- > -- > Paul Scherrer Institut > Dr. Tim Gruene > - persoenlich - > Principal Investigator > Biology and Chemistry > OFLC/102 > CH-5232 Villigen PSI > > Phone: +41 (0)56 310 5297 > > GPG Key ID = A46BEE1A >
